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Using Optical Sensors for Bioprocess Monitoring: A Measurement Technique for...
Figure 1: Contact-free measurement with chemical optical sensors Over the past decade, the application of chemical optical sensors for bioprocess monitoring has gradually taken roots. Constant further...
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Monitoring Live Biomass in Disposable Bioreactors
Photo 1: Original disposable Aber Instruments biomass probe attached to light-weight preamplifier Often simply referred to as capacitance, radio-frequency (RF) impedance has been used for over two...
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Bioreactor Manufacturing Platforms for Cell Therapies
2,000-L stainless steel bioreactor As an increasing number of cell therapies move into late-phase trials, developers are considering innovative solutions to address scale-up and commercialization...
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Performance Qualification of a Single-Use, Stirred-Tank Bioreactor with CHO-S...
Photo 1: Pall Allegro STR 200 bioreactor The increasing role and importance of cell culture in biophamaceutical manufacturing has led to considerable research and development (R&D) into bioreactor...
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Continuous Cell Culture Operation at 2,000-L Scale
In the biopharmaceutical industry, continuous manufacturing is often cited as a method for increasing the productivity of bioprocesses (1). Compared with batch processing, it has the potential to...
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Design and Performance of Single-Use, Stirred-Tank Bioreactors
Figure 1: Sterilizing filter systems Single-use components and systems have been incorporated into many bioprocesses as an alternative to cleanable, reusable systems. A wide range of publications have...
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Simplification of Fed-Batch Processes with a Single-Feed Strategy
Figure 1: Traditional feeding strategies rely on a main feed and a separate alkaline feed containing l-tyrosine and l-cysteine (A). This study demonstrates the use of a combined main feed with high...
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Platform Solutions for Cell Therapy Manufacturing
Figure 1: Efficiency of Lonza bioreactor platform over traditional 2D planar culture methods Advances in cell therapy have resulted in significant progress toward treating some widespread and difficult...
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Aggregation from Shear Stress and Surface Interaction: Molecule-Specific or...
WWW.PHOTOS.COM Exposure to solid–liquid and air–water interfaces during production, freezing and thawing, shipment and storage of protein therapeutics may be a contributing factor in their degradation...
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Difficult-to-Express Proteins: Resolving Bioprocessing Challenges with a...
Figure 1: AcuSyst hollow-fiber bioreactors; electron microscopy images of cells growing around hollow fibers within an AcuSyst bioreactor; cells grow to high densities in the extra capillary (EC) space...
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Process Development of Microbial Plasmid DNA: Fast-Tracking with Modular...
Photo 1: ambr 250 modular automated minibioreactor system There has been a rapid rise in the number of positive clinical outputs from clinical studies based on gene and cell therapies. This is in...
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The Unican Concept: Engineering Dual Capability into Single-Use Vessels
Use of disposable bioreactors in the biopharmaceutical industry has increased gradually over the past several years in pilot, clinical, and production scale facilities (1–4). Reduced time to market in...
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A Stirred, Single-Use, Small-Scale Process Development System: Evaluation for...
Photo 1: An ambr 250 modular eight-bioreactor system Mammalian and microbial protein production platforms have been used for over 30 years to produce a number of successful biologic drugs, including...
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Simplify Upstream Process Development and Scale-Up: Single-Use 5:1...
Figure 1: Crossflow sparger (with a 0.02-VVM frit) CO2 stripping performance in a 500-L SUB Single-use technologies (SUTs) have been adopted widely in the biopharmaceutical industry for product...
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Enhanced Galactosylation of Monoclonal Antibodies: Using Medium Supplements...
Figure 1: Electropherogram of IgG MAb 1 glycans using the HTP method The biopharmaceutical industry needs better understanding of how monoclonal antibody (MAb) glycosylation is influenced by components...
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Reducing Variability in Cell-Specific Productivity in Perfusion Culture: A...
Figure 1A: Flow schematic of the cell expansion process; see text for details regarding process steps (numbered 1–6). Colored (upward-pointing) arrows indicate location (between steps 4 and 5) where...
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Cell Expansion with Dissolvable Microcarriers
Figure 1: Demonstration of hMSC attachment to Corning Synthemax II–coated dissolvable microcarriers; (A) hMSCs and Synthemax II–coated dissolvable microcarriers during the cell attachment phase at 0...
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Single-Use Bioreactors: Performance and Usability Considerations Part 1:...
Figure 1: Highlights of requirements and features to be considered during single-use bioreactor selection and evaluation process; (A) highlight of key concepts for consideration; (B) features of the...
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Demonstrating Scalable T-Cell Expansion in Stirred-Tank Bioreactors
PHOTO BY ERIC SCHOLLENBERGER Emerging cell therapies have excited the pharmaceutical industry because they indicate potential new pathways to treat some of the most life-threatening diseases. T-cell...
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Oxygen Mass Transfer Correlation for a Rocking-Motion Bioreactor System
Figure 1: Example of dissolved oxygen (DO) profile over the course of one test: (1) stop rocking and compress to eliminate headspace air; (2) inflate with nitrogen; (3) start rocking; (4) stop rocking...
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